Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transgenic hookworm secretes anti-tetrodotoxin human single chain antibody.

Nature communications·2026
Same author

Serotonin signaling modulates growth and motility in juvenile Fasciola hepatica.

PLoS neglected tropical diseases·2026
Same author

Simplifying Daily Cortisol Cycle Analysis: Validation and Benchmarking of the Cortisol Sine Score Against Cosinor and JTK_CYCLE models.

medRxiv : the preprint server for health sciences·2026
Same author

Meclonazepam sensitivity of parasitic flatworms correlates with TRPM<sub>MCLZ</sub> sensitivity to meclonazepam.

International journal for parasitology. Drugs and drug resistance·2026
Same author

Targeting the lung innate pathways during tuberculosis can improve vaccine-induced protection via Th17 responses in diversity outbred mice.

mBio·2026
Same author

Single-cell transcriptome atlas of adult male and female human hookworm <i>Ancylostoma ceylanicum</i>.

iScience·2025

Related Experiment Video

Updated: Jun 1, 2026

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
06:54

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Published on: March 9, 2022

RNAi effector diversity in nematodes.

Johnathan J Dalzell1, Paul McVeigh, Neil D Warnock

  • 1Molecular Biosciences-Parasitology, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.

Plos Neglected Tropical Diseases
|June 14, 2011
PubMed
Summary
This summary is machine-generated.

Parasitic nematodes show variable susceptibility to RNA interference (RNAi) gene silencing. This study found no evidence of RNAi effector deficiencies in parasitic nematodes, suggesting RNAi is broadly applicable for gene function studies in these organisms.

More Related Videos

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
09:40

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
11:22

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Published on: February 13, 2013

Related Experiment Videos

Last Updated: Jun 1, 2026

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
06:54

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Published on: March 9, 2022

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
09:40

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
11:22

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Published on: February 13, 2013

Area of Science:

  • Molecular Biology
  • Genetics
  • Parasitology

Background:

  • RNA interference (RNAi) is a key tool for gene function studies in helminths.
  • Parasitic nematodes exhibit variable susceptibility to RNAi, hindering research.
  • Understanding the molecular basis of this variability is crucial for advancing nematode genetics.

Purpose of the Study:

  • To investigate inter-species differences in RNAi effector complements among nematodes.
  • To determine if variations in RNAi pathway proteins explain differential susceptibility in parasitic nematodes.
  • To assess the conservation of RNAi machinery across diverse nematode species.

Main Methods:

  • Performed a primary sequence similarity survey for 77 Caenorhabditis elegans RNAi pathway proteins.
  • Analyzed genomic or transcriptomic datasets from 13 nematode species, including parasitic and free-living types.
  • Verified all identified orthologs through domain-structure analysis.

Main Results:

  • Key proteins for double-stranded RNA (dsRNA) uptake and spread are largely absent in parasitic nematodes.
  • Argonaute proteins (AGOs) involved in gene regulation are conserved, but those for exogenous dsRNA induction are not.
  • Secondary Argonautes (SAGOs) and the nuclear AGO NRDE-3 are poorly conserved or absent in parasites; Caenorhabditis species have expanded RNAi repertoires.

Conclusions:

  • No specific RNAi effector deficiencies were identified that correlate with reduced susceptibility in parasitic nematodes.
  • Despite quantitative differences in RNAi components, functional protein groups are qualitatively conserved across species.
  • The findings support the broad applicability of RNAi as a genetic tool for studying both RNAi-refractory and RNAi-competent nematode parasites.