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Related Experiment Videos

Synaptic membrane aging in the central nervous system.

S Ando1, Y Tanaka

  • 1Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.

Gerontology
|January 1, 1990
PubMed
Summary
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Neuronal membrane potentials decrease with aging due to reduced phosphatidylcholine and Na+,K(+)-ATPase activity. This impacts neuronal excitability and transmitter uptake in aging mice.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Gerontology

Background:

  • Neuronal membrane potential is crucial for brain function, influencing excitability and neurotransmitter transport.
  • Aging is associated with cellular and molecular changes that can affect neuronal function.
  • Synaptosomes are valuable models for studying neuronal membrane properties and associated enzyme activities.

Purpose of the Study:

  • To investigate age-related changes in synaptosomal membrane potential in mice.
  • To elucidate the mechanisms underlying alterations in neuronal membrane potential during senescence.
  • To assess the role of Na+,K(+)-ATPase activity and phosphatidylcholine content in age-dependent membrane potential shifts.

Main Methods:

  • Preparation of synaptosomes from mice of different age groups.

Related Experiment Videos

  • Measurement of synaptosomal resting membrane potential.
  • Assay of Na+,K(+)-ATPase activity.
  • Quantification of phosphatidylcholine content.

    • Main Results:

    • Synaptosomal resting membrane potential significantly decreased in aged mice (senescence).
    • A concomitant decrease in Na+,K(+)-ATPase activity was observed in senescent synaptosomes.
    • Reduced phosphatidylcholine content was associated with decreased Na+,K(+)-ATPase activity in early senescence.
    • In late senescence, reduced Na+,K(+)-ATPase enzyme content appeared to be the primary cause of decreased activity and membrane potential.

    Conclusions:

    • Aging leads to a significant decline in synaptosomal membrane potential in mice.
    • Decreased Na+,K (+)-ATPase activity, influenced by both lipid environment (phosphatidylcholine) and enzyme content, contributes to age-related membrane potential changes.
    • These alterations in membrane potential may underlie age-associated deficits in neuronal excitability and function.