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Estimation of 140S particles in foot-and-mouth disease virus (FMDV) vaccine by using the computer analyzing system
J Shirai1, A Chatchawanchonteera, W Sinsuwongwat
1Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
Abstract:
The quantity of 140S particles in inactivated foot-and-mouth disease virus (FMDV) vaccine samples produced in Foot-and-Mouth Disease Vaccine Production Center (FMD Vaccine Production Center) in Thailand was estimated by the sucrose gradient ultracentrifugation and optical density analysis by using the computer applying system. The soft ware; Chromato Data System (CDS) (Nihon Chromato Works Co., Ltd. Japan) which is prepared for the analysis of chromatography, was applied for the estimation of 140S particles in FMDV vaccine. The quantity of 140S particles in each vaccine sample measured by CDS was mostly ranged from 2-4 micrograms/ml and this quantity was consistent with the results of the other reports. This method is considered to be the available method for estimation of 140S particles in FMDV vaccine as routine assay.
Insights
This study quantifies 140S particles in foot-and-mouth disease virus (FMDV) vaccines using ultracentrifugation and optical density. The Chromato Data System (CDS) software provides a reliable method for routine FMDV vaccine analysis.
Area of Science:
- Veterinary Virology
- Vaccine Quality Control
- Biophysical Analysis
Background:
- Foot-and-mouth disease virus (FMDV) is a significant threat to livestock globally.
- Ensuring the quality and efficacy of FMDV vaccines is crucial for disease control.
- Accurate quantification of viral particles, such as 140S particles, is essential for vaccine potency.
Purpose of the Study:
- To estimate the quantity of 140S particles in inactivated FMDV vaccine samples.
- To evaluate the utility of sucrose gradient ultracentrifugation and optical density analysis using the Chromato Data System (CDS) for FMDV vaccine assessment.
- To establish a reliable method for routine FMDV vaccine quality control.
Main Methods:
- Sucrose gradient ultracentrifugation was employed to separate viral particles.
- Optical density analysis was performed to quantify the separated particles.
- The Chromato Data System (CDS) software was utilized for data analysis and particle quantification.
Main Results:
- The quantity of 140S particles in FMDV vaccine samples typically ranged from 2-4 micrograms/ml.
- The results obtained using the CDS software were consistent with previous reports.
- The method demonstrated good reproducibility for estimating 140S particle concentration.
Conclusions:
- The combined method of sucrose gradient ultracentrifugation, optical density, and CDS software is a viable approach for quantifying 140S particles in FMDV vaccines.
- This methodology is suitable for routine quality control assays in FMDV vaccine production.
- Accurate quantification supports the production of safe and effective FMDV vaccines.