Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

What is Genetic Engineering?

What is Genetic Engineering?

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Virus-mediated, heritable gene editing in groundcherry (<i>Physalis grisea</i>).

Frontiers in plant science·2026

Same author

At the breaking point: developmental and molecular insights into <i>Physalis grisea</i> fruit abscission.

Frontiers in plant science·2026

Same author

A neofunctionalized flowering antagonist created an evolutionary contingency that channeled Solanaceae adaptation.

bioRxiv : the preprint server for biology·2026

Same author

Solanum pan-genetics reveals paralogues as contingencies in crop engineering.

Nature·2025

Same author

PlantGENE: Advancing plant transformation through community engagement.

The Plant journal : for cell and molecular biology·2025

Same author

Convergent evolution of plant prickles by repeated gene co-option over deep time.

Science (New York, N.Y.)·2024

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

Engineering virus resistance using a modified potato gene.

Jason Cavatorta¹, Kari W Perez, Stewart M Gray

¹Department of Plant Breeding, Cornell University, Ithaca, NY, USA.

Plant Biotechnology Journal

|June 15, 2011

Summary

Transgenic potato plants expressing a modified eIF4E gene show resistance to Potato virus Y (PVY). This intragenic approach engineers virus resistance by altering a key step in the viral infection process.

More Related Videos

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

Published on: February 28, 2025

Related Experiment Videos

Last Updated: Jun 1, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

Published on: February 28, 2025

Area of Science:

Plant pathology
Molecular biology
Genetics

Background:

Potyviruses cause significant damage to potato crops worldwide.
Natural mutations in translation initiation factor eIF4E confer potyvirus resistance in many plant species.
No eIF4E-mediated resistance genes have been identified in potato to date.

Purpose of the Study:

To investigate the potential of eIF4E-mediated resistance in potato against Potato virus Y (PVY).
To engineer a de novo allele for PVY resistance in potato using site-directed mutagenesis of the susceptible ortholog.
To assess the efficacy and agronomic impact of the engineered resistance.

Main Methods:

Transgenic expression of the pepper pvr1(2) gene in potato.
Site-directed mutagenesis of the potato eIF4E ortholog to create a resistance allele.
Overexpression of the mutated potato allele in potato plants.
Evaluation of PVY resistance, eIF4E expression levels, and plant/tuber phenotypes.

Main Results:

Transgenic expression of pepper pvr1(2) conferred PVY resistance in potato.
Overexpression of the site-directed mutated potato eIF4E allele resulted in PVY resistance.
Resistant potato lines exhibited high eIF4E mRNA and protein levels.
Resistant plants maintained normal growth and produced phenotypically similar tubers.

Conclusions:

Engineered intragenic resistance using modified potato eIF4E is effective against PVY.
This approach offers a potentially more acceptable method for developing virus-resistant crops.
The strategy may be applicable to other plant-viral pathosystems for engineering broad-spectrum resistance.