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Updated: Jun 1, 2026

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
Published on: March 5, 2017
The maize mutant barren stalk1 is defective in axillary meristem development
Matthew K Ritter1, Christopher M Padilla, Robert J Schmidt
1Section of Cell and Developmental Biology, Division of Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093-0116 USA.
Abstract:
barren stalk1 is a recessive mutant of maize that has no tassel branches, spikelets, tillers, or ears. Here we present a detailed characterization of the ba1 mutant phenotype, including scanning electron microscopy of developing inflorescences, in situ hybridization analysis using a meristem marker, molecular mapping, and genetic analysis demonstrating an epistatic relationship between ba1 and teosinte branched1 (tb1). These data show that the primary defect in the ba1 mutant is a failure in axillary meristem development.
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