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Related Experiment Videos

Complement activation and white cell sequestration in postischemic skeletal muscle.

B B Rubin1, A Smith, S Liauw

  • 1Division of Vascular Surgery, Toronto General Hospital, Ontario, Canada.

The American Journal of Physiology
|August 1, 1990
PubMed
Summary
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Skeletal muscle reperfusion injury involves white blood cells (WBCs) and complement activation. This study found that the alternative complement pathway and immediate WBC sequestration occur after ischemia-reperfusion.

Area of Science:

  • Biomedical Science
  • Physiology
  • Immunology

Background:

  • Skeletal muscle ischemia leads to reperfusion injury, where tissue damage is worsened.
  • White blood cells (WBCs) and complement proteins are implicated in this reperfusion injury process.

Purpose of the Study:

  • To investigate the activation of classical and alternative complement pathways.
  • To quantify white blood cell (WBC) sequestration in postischemic skeletal muscle.
  • To define the timeline of these events within the first 48 hours of reperfusion in vivo.

Main Methods:

  • Utilized an isolated canine gracilis muscle model for in vivo study.
  • Measured systemic levels of complement factors B (alternative pathway) and C4 (classical pathway) via hemolytic assay.
  • Assessed WBC sequestration using arterial-venous WBC differences and tissue myeloperoxidase activity.

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Main Results:

  • Systemic levels of factor B (alternative pathway) decreased by 18%, while C4 levels (classical pathway) showed no consistent change.
  • Significant WBC sequestration was observed within the first 4 hours of reperfusion.
  • Tissue myeloperoxidase activity, an indicator of WBC infiltration, was markedly elevated (97-fold) after 48 hours.

Conclusions:

  • Skeletal muscle ischemia-reperfusion activates the alternative complement pathway but not the classical pathway.
  • Immediate and sustained sequestration of WBCs occurs in postischemic skeletal muscle.
  • These findings highlight the roles of alternative complement activation and WBCs in skeletal muscle reperfusion injury.