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13C and 31P NMR studies of the pentose phosphate pathway in human erythrocytes
P W Kuchel1, H A Berthon, W A Bubb
1Department of Biochemistry, University of Sydney, NSW, Australia.
Biomedica Biochimica Acta
|January 1, 1990
Abstract:
31P NMR was used to monitor the concentrations of some of the intermediates of the non-oxidative pentose phosphate pathway (PPP) during the dissimilation of inosine and phosphate in dilute haemolysates. The temperature dependence of the ketone/hydrate ratio in dihydroxyacetone phosphate and the relative proportions of the isomers of sedoheptulose 1,7-bisphosphate were measured. The 31P NMR time courses were compared with the simulations obtained with a computer model of the modified F-type PPP.