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Identification of ultraviolet-inducible proteins that bind to a TGACAACA sequence in the polyoma virus regulatory

Z A Ronai1, I B Weinstein

  • 1Comprehensive Cancer Center, Columbia University, New York, New York 10032.

Cancer Research
|September 1, 1990
PubMed

Insights

Exposure to UV light induces DNA-binding proteins in mammalian cells that respond to DNA damage. These proteins may play a role in cellular defense and carcinogenesis.

Area of Science:

  • Molecular biology
  • Cellular response to DNA damage
  • Carcinogenesis

Background:

  • Mammalian cells exhibit defense mechanisms against DNA damage from agents like UV light.
  • These responses are crucial for cellular integrity and can influence cancer development.

Purpose of the Study:

  • To identify DNA-binding proteins induced by UV radiation in mammalian cells.
  • To characterize the binding specificity and molecular properties of these UV-induced proteins.

Main Methods:

  • Utilized a polyoma virus DNA fragment containing replication origin and regulatory regions.
  • Employed UV-irradiated normal rat fibroblasts.
  • Applied UV response element affinity chromatography for protein purification.

Main Results:

  • Identified a set of DNA-binding proteins induced 6-24 hours post-UV exposure.
  • These proteins bind to a specific octamer sequence (TGACAACA), termed the "UV response element."
  • The major purified protein (40,000 MW) co-purifies with c-fos but not c-jun proteins.

Conclusions:

  • UV-induced proteins binding to the UV response element are identified.
  • These proteins may mediate cellular responses to DNA damage.
  • Potential role in cellular defense and carcinogenesis warrants further investigation.

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