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Updated: Jun 1, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

Normal prostate-derived stromal cells stimulate prostate cancer development.

Yu-Bing Peng¹, Juan Zhou, Yan Gao

¹Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

|June 16, 2011

Summary

This summary is machine-generated.

Normal prostate stromal cells (PSCs) significantly enhance prostate cancer growth and metastasis by promoting tumor cell proliferation and migration. PSCs regulate key genes involved in cell cycle and mobility, driving tumor progression.

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Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture

Published on: November 22, 2019

Area of Science:

Oncology
Cancer Biology
Prostate Cancer Research

Background:

Stromal cells are critical regulators of tumor progression.
The specific role of normal prostate-derived stromal cells (PSCs) in prostate cancer development requires further elucidation.

Purpose of the Study:

To investigate the significance of normal prostate-derived stromal cells (PSCs) in prostate cancer development.
To analyze the impact of PSCs on tumor growth, proliferation, apoptosis, and androgen receptor expression in vivo and in vitro.

Main Methods:

Establishment of an in vivo subcutaneous tumor model using DU145 cells with and without PSCs, including comparisons in pre-castrated mice.
Immunohistochemical analysis to assess proliferation, apoptosis, and androgen receptor expression.
In vitro assessment of DU145 cell viability and migratory capability.
cDNA microarray analysis to identify differentially expressed genes in DU145 cells.

Main Results:

Tumors were only detectable in groups with DU145 cells, with significantly more aggressive growth when co-injected with PSCs (Group 2 vs. Group 1).
Group 2 exhibited significantly higher proliferation compared to Group 1, with no significant differences in apoptosis or androgen receptor expression.
In vitro, DU145 cells from Group 2 showed higher viability and migratory capability than those from Group 1.
Differential gene expression was observed in DU145 cells, particularly genes regulating cell cycle and cell mobility (e.g., GADD45A, RHOV, KLK11, PCK1).

Conclusions:

Normal prostate-derived stromal cells potentiate prostate tumor growth in vivo.
PSCs influence tumor development, at least partly, by regulating cell-cycle and migration-related gene expression within tumor cells.
These findings highlight the crucial role of the tumor microenvironment, specifically stromal cells, in prostate cancer progression.