Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Norepinephrine evokes a marked Mg2+ efflux from liver cells.

A Romani1, A Scarpa

  • 1Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106.

FEBS Letters
|August 20, 1990
PubMed
Summary

Norepinephrine triggers magnesium (Mg2+) efflux from liver cells via beta-adrenergic receptors, mediated by cyclic AMP (cAMP). This cellular response highlights a key pathway for magnesium regulation in hepatocytes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sociodemographic profile, health-related behaviours and experiences of healthcare access in Italian transgender and gender diverse adult population.

Journal of endocrinological investigation·2024
Same author

Estimate and needs of the transgender adult population: the SPoT study.

Journal of endocrinological investigation·2024
Same author

Search for Dark-Matter-Nucleon Interactions via Migdal Effect with DarkSide-50.

Physical review letters·2023
Same author

Search for Dark Matter Particle Interactions with Electron Final States with DarkSide-50.

Physical review letters·2023
Same author

Effects of hormonal treatment on dermatological outcome in transgender people: a multicentric prospective study (ENIGI).

Journal of endocrinological investigation·2022
Same author

Apparent autistic traits in transgender people: a prospective study of the impact of gender-affirming hormonal treatment.

Journal of endocrinological investigation·2022

Area of Science:

  • Cellular Physiology
  • Hormonal Regulation
  • Ion Transport

Background:

  • Magnesium (Mg2+) is crucial for cellular functions.
  • Hormonal influences on intracellular magnesium levels are not fully understood.
  • Hepatocytes play a central role in metabolic homeostasis.

Purpose of the Study:

  • To investigate the effect of adrenergic stimulation on magnesium (Mg2+) transport in rat liver cells.
  • To elucidate the specific receptor pathways and intracellular mechanisms involved in Mg2+ efflux.

Main Methods:

  • Perfusion of rat livers with norepinephrine.
  • Isolation and treatment of hepatocytes with various agonists and antagonists.
  • Measurement of magnesium (Mg2+) efflux and influx.

Related Experiment Videos

Main Results:

  • Norepinephrine induced a dose-dependent Mg2+ efflux from perfused livers and isolated hepatocytes.
  • Beta-adrenergic receptor antagonists blocked the Mg2+ efflux, while alpha-antagonists did not.
  • Forskolin and cAMP analogues mimicked the Mg2+ efflux.
  • Carbachol and vasopressin induced Mg2+ influx in hepatocytes.

Conclusions:

  • Beta-adrenergic receptor activation triggers significant Mg2+ efflux from liver cells.
  • The Mg2+ efflux is mediated by an increase in intracellular cyclic AMP (cAMP) levels.
  • This pathway represents a novel mechanism for regulating cellular magnesium homeostasis.