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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 1, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Intron cleavage affects processing of alternatively spliced transcripts.

Tibor Pastor1, Andrea Dal Mas, Gabriele Talotti

  • 1International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149 Trieste, Italy.

RNA (New York, N.Y.)
|June 16, 2011
PubMed
Summary

Intronic cleavage events using ribozymes (Rz) can alter alternative splicing. However, intron integrity is crucial for efficient pre-mRNA biosynthesis, even if not strictly required for splicing itself.

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Gene Regulation

Background:

  • Alternative splicing is a key mechanism for generating diverse mRNA transcripts from a single gene.
  • Ribozymes (Rz) are RNA molecules with catalytic activity that can mediate RNA cleavage.
  • Intronic elements play critical roles in regulating pre-mRNA processing and splicing decisions.

Purpose of the Study:

  • To investigate the impact of intronic cotranscriptional cleavage events on alternative pre-mRNA processing.
  • To compare the effects of different ribozymes and Microprocessor target sequences (MTSs) on alternative splicing.
  • To determine the necessity of intron integrity for efficient pre-mRNA biosynthesis.

Main Methods:

  • Utilized a fibronectin EDA minigene system to study alternative splicing.
  • Introduced various hammerhead ribozymes (Rz) and hepatitis δ ribozymes into intronic positions.
  • Assessed the cleavage efficiency of ribozymes and Microprocessor target sequences (MTSs).
  • Analyzed the effects on alternative splicing, exon definition, and mature mRNA levels.

Main Results:

  • Intronic MTSs showed inefficient cleavage and did not impact alternative splicing or transcript levels.
  • Hammerhead Rz derivatives and hepatitis δ Rz were efficiently cleaved, significantly affecting alternative splicing.
  • Despite efficient ribozyme-mediated cleavage, mature mRNA levels were reduced to approximately 40%.
  • This reduction in mature transcripts was independent of Rz type, intronic position, alternative splicing, or exon definition.

Conclusions:

  • Efficient intronic cleavage by ribozymes can modulate alternative splicing.
  • Intron integrity is essential for efficient pre-mRNA biosynthesis, although not strictly required for the splicing process itself.
  • These findings highlight a novel regulatory role for introns beyond their direct involvement in splicing catalysis.