Renal perfusion: noninvasive measurement with multidetector CT versus fluorescent microspheres in a pig model

To validate the measurement of renal perfusion with multidetector computed tomography (CT) with a low-rate injection of contrast medium (ie, 3 mL/sec) through a catheter placed peripherally with gamma variate extended modeling in a pig model, compared with a reference method of fluorescent microspheres.

This study was approved by the Institutional Animal Care and Use Committee. Renal perfusion was measured in 10 anesthetized pigs simultaneously with multidetector CT and with fluorescent microspheres, which are the reference standard for measuring regional renal perfusion. In each pig, measurements were obtained under three conditions. These were dopamine infusion, dopamine infusion with vascular expansion, and angiotensin II infusion. Aortic and cortical time-attenuation curves were modeled to measure renal perfusion with the gamma variate model. The renal perfusion measurements with the multidetector CT and that with microspheres were compared with least squares regression analysis and Bland-Altman plots.

Perfusion as measured with multidetector CT and that as measured with microspheres were strongly correlated (ρ = 0.93, P < .0001). Multidetector CT renal perfusion with dopamine infusion (3.13 mL/min/g ± 0.53) was not changed after volume expansion (3.37 mL/min/g ± 0.75, P = .35) but was significantly decreased after angiotensin II injection (2.01 mL/min/g ± 0.57, P = .0001).

Multidetector CT provides reliable measurements of single-kidney perfusion with peripheral low-rate contrast medium injection.