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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: Jun 1, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Proteomic analysis and discovery using affinity proteomics and mass spectrometry.

Niclas Olsson1, Christer Wingren, Mikael Mattsson

  • 1Department of Immunotechnology, Lund University, Lund, Sweden, and CREATE Health, BMC D13, Lund, Sweden.

Molecular & Cellular Proteomics : MCP
|June 16, 2011
PubMed
Summary

This study introduces a novel global proteome survey method using motif-specific antibodies. This approach enables species-independent proteome profiling with fewer antibodies, overcoming limitations in current affinity-proteomic techniques.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Proteomics
  • Immunology
  • Biochemistry

Background:

  • Antibody-based microarrays are advanced affinity-proteomic tools with clinical potential.
  • Current array resolution is limited by the number of available, well-characterized antibodies, posing a bottleneck.
  • A need exists for methods that can analyze proteomes broadly without requiring a vast antibody library.

Purpose of the Study:

  • To present a new, species-independent method for proteome analysis using a limited antibody set.
  • To overcome the antibody availability bottleneck in high-resolution proteomic studies.
  • To demonstrate the feasibility of the global proteome survey approach.

Main Methods:

  • Utilized context-independent-motif-specific antibodies targeting short amino acid sequences.
  • Enriched motif-containing peptides from digested proteomes using these antibodies.
  • Detected and identified enriched peptides via mass spectrometry.

Main Results:

  • Successfully profiled human colon tissue, yeast cell lysate, and mouse liver tissue.
  • Demonstrated proof-of-concept for the global proteome survey method.
  • Showcased the ability to probe diverse proteomes with a limited antibody repertoire.

Conclusions:

  • The global proteome survey offers a novel, efficient strategy for broad proteomic analysis.
  • This method circumvents the limitations of antibody availability in high-throughput proteomics.
  • The approach holds promise for diverse proteomic applications across species.