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Updated: Jun 1, 2026

11:10
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
Published on: December 27, 2010
Frequency of CXCR4-using viruses in primary HIV-1 infections using ultra-deep pyrosequencing
Stéphanie Raymond1, Adrien Saliou, Florence Nicot
1INSERM U, Toulouse, France.
AIDS (London, England)
|June 16, 2011
Abstract:
We used ultra-deep pyrosequencing and the Toulouse Tropism Test phenotypic assay to determine the prevalence of CXCR4-using viruses in 21 patients with primary HIV-1 infections. We found X4-containing virus populations in 9% of patients by ultra-deep pyrosequencing using position-specific scoring matrices (PSSM(X4/R5)) or geno2pheno(5.75) and in 14% using the combined 11/25 and net charge rule. The phenotypic assay identified 9% of CXCR4-using viruses. This confirms that R5 viruses are predominant in primary HIV-1 infections.

