Related Experiment Video
Updated: Jun 1, 2026
11:28
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
Published on: April 28, 2015
Polyethylenimine bioconjugates for imaging and DNA delivery in vivo
Andrea Masotti1, Francesco Pampaloni
1Gene Expression-Microarrays Laboratory, IRCCS-Bambino Gesù Children's Hospital, Rome, Italy. andrea.masotti@opbg.net
Methods in Molecular Biology (Clifton, N.J.)
|June 16, 2011
Abstract:
Polyamine polymers are among the commonest polymers used in biomedicine. Among polyamine -polymers, polyethylenimine (PEI) may be used as an efficient delivery vehicle for nucleic acids (DNA, RNA, etc.) or employed as a versatile imaging probe in vivo. In this chapter, the preparation of various PEI bioconjugates will be fully explained and discussed.
Related Concept Videos
01:24Site-Targeted Drug Delivery Systems: Polymeric Carriers
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...