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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jun 1, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Site-specific DNA labeling by Staudinger ligation.

Samuel H Weisbrod, Anna Baccaro, Andreas Marx

    Methods in Molecular Biology (Clifton, N.J.)
    |June 16, 2011
    PubMed
    Summary

    This study introduces a novel method for site-specific DNA labeling using Staudinger ligation. Azido-modified DNA can be efficiently labeled with phosphines under mild conditions, enabling precise molecular tagging.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Organic Chemistry

    Background:

    • Site-specific and chemoselective DNA labeling presents significant challenges in molecular biology.
    • Bioorthogonal reactions offer a pathway for labeling biomolecules under physiological conditions.

    Purpose of the Study:

    • To develop a protocol for site-specific DNA labeling utilizing the Staudinger ligation.
    • To enable precise incorporation and subsequent labeling of modified DNA strands.

    Main Methods:

    • Synthesis of an azido-TTP (triphosphate) analogue.
    • Enzymatic incorporation of azido-modified nucleotides into DNA.
    • Synthesis of complementary phosphine labels.
    • Staudinger ligation for labeling azido-DNA with biotin-phosphine.

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    Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

    Published on: August 26, 2009

    Related Experiment Videos

    Last Updated: Jun 1, 2026

    Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
    12:07

    Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

    Published on: November 22, 2014

    Robust 3D DNA FISH Using Directly Labeled Probes
    12:16

    Robust 3D DNA FISH Using Directly Labeled Probes

    Published on: August 15, 2013

    Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
    14:36

    Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

    Published on: August 26, 2009

    Main Results:

    • Successful synthesis of an azido-TTP analogue.
    • Enzymatic incorporation of azido-TTP into DNA strands.
    • Demonstration of Staudinger ligation for DNA labeling with biotin-phosphine.
    • Achieved approximately 70% conversion in the labeling reaction.

    Conclusions:

    • The Staudinger ligation is a viable and efficient method for site-specific DNA labeling.
    • This protocol facilitates the development of novel tools for DNA analysis and modification.
    • The bioorthogonal nature of the reaction allows for mild and selective labeling.