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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
14:44

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 15, 2014

A novel small-subunit ribosomal protein of yeast mitochondria that interacts functionally with an mRNA-specific

T W McMullin1, P Haffter, T D Fox

  • 1Section of Genetics and Development, Cornell University, Ithaca, New York 14853-2703.

Molecular and Cellular Biology
|September 1, 1990
PubMed

Insights

The PET123 protein is a mitochondrial ribosomal protein crucial for yeast translation. This finding supports a model where PET122 interacts with the ribosome to initiate coxIII mRNA translation.

Area of Science:

  • Mitochondrial biology
  • Molecular genetics
  • Protein synthesis

Background:

  • Mitochondrial translation of cytochrome c oxidase subunit III (coxIII) mRNA in yeast requires nuclear-encoded activator proteins.
  • Mutations in PET122, a translational activator, can be suppressed by mutations in PET123.
  • PET123 is essential for the translation of all mitochondrial gene products.

Purpose of the Study:

  • To characterize the function and localization of the PET123 protein.
  • To investigate the relationship between PET122 and PET123 in mitochondrial translation.

Main Methods:

  • Generation of an antibody against the PET123 protein.
  • Immunological detection and quantification of PET123.
  • Analysis of PET123 accumulation in relation to mitochondrial genes.

Main Results:

  • PET123 was identified as a mitochondrial ribosomal protein of the small subunit.
  • PET123 levels are comparable to other mitochondrial ribosomal proteins.
  • PET123 accumulation depends on the presence of the 15S rRNA gene.

Conclusions:

  • PET123 is a component of the yeast mitochondrial small ribosomal subunit.
  • The findings support a model where PET122 interacts with the small ribosomal subunit to facilitate coxIII mRNA translation initiation.

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