A bioconjugated phospholipid polymer biointerface with nanometer-scaled structure for highly sensitive immunoassays

This method relates to the preparation of a phospholipid polymer platform and the immobilization of an antibody as a bioaffinity ligand onto the platform to construct a biointerface for highly sensitive immunoassays. The specific phospholipid polymer used in this work is poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-p-nitrophenyloxycarbonyl poly(ethylene glycol) methacrylate (MEONP)] (PMBN). The PMBN surface could immobilize specific antibodies through covalent chemical bonding by the reaction between MEONP units and amino groups in the antibody. In addition, the PMBN surface could prevent nonspecific protein adsorption from an analyte sample without the use of blocking reagents based on the fundamental properties of the MPC units. Furthermore, a nanometer-scaled particle deposition surface is constructed with PMBN by an electrospray deposition method to enhance the sensitivity by increasing the overall surface area of the biointerface.