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Updated: Jun 1, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Cytotoxic triterpenoids from Azadirachta indica
JinXiong Chen1, JianChao Chen, Yun Sun
1Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, P. R. China.
Abstract:
Two new tirucallane triterpenoids, 24,25-epoxy-3 β-hydroxy-20-oxo-7-tirucallene (1) and 22,23;24,25-diepoxy-3 β-hydroxy-7-tirucallene (2), and a new tetranortriterpenoid, 4 α-hydroperoxy-6- O-acetylnimbandiol (3), along with eight known compounds, were isolated from the branches and leaves of Azadirachta indica. Their structures were elucidated through spectroscopic and chemical methods. The cytotoxic assay showed that the abundant constituent nimbolide (8) had obvious cytotoxic activities against HL-60, SMMC7721, A549, MCF-7, and SW-480 cell lines, with IC₅₀ values of 0.8 ± 0.1, 2.2 ± 0.2, 1.9 ± 1.3, 4.5 ± 1.1, and 2.3 ± 0.1 µM, respectively.
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