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Orthopaedic specimen preparation: what pathologists should know and do

Michael J Klein1, Vincent A Memoli

  • 1Department of Pathology and Laboratory Medicine, Hospital for Special Surgery, Weill College of Medincine of Cornell Universiy, New York, New York 10021, USA. kleinm@hss.edu

Seminars in Diagnostic Pathology
|June 17, 2011
PubMed

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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...