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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
Recombinant DNA01:09

Recombinant DNA

Overview

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Related Experiment Video

Updated: Jun 1, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Recombinase-mediated gene stacking as a transformation operating system.

David W Ow1

  • 1South China Botanical Garden, Guangzhou 510650, China. dow@scbg.ac.cn

Journal of Integrative Plant Biology
|June 17, 2011
PubMed
Summary

Combining multiple transgenes into a single genome efficiently is crucial for crop improvement. This study explores using site-specific recombinases for precise gene stacking, simplifying the development of advanced transgenic crops.

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Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

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Last Updated: Jun 1, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

Area of Science:

  • Plant Biotechnology
  • Molecular Genetics
  • Crop Science

Background:

  • Traditional methods for combining transgenes involve independent loci, requiring large populations to achieve desired trait combinations.
  • Increasing transgene numbers necessitates larger populations, complicating the development of elite cultivars with multiple engineered traits.

Purpose of the Study:

  • To explore efficient strategies for combining multiple transgenes into a single genome.
  • To investigate the use of site-specific recombinases as a method for gene stacking.

Main Methods:

  • Discusses gene stacking via site-specific integration.
  • Highlights the use of host-dependent homology-based processes and heterologous site-specific recombination systems.

Main Results:

  • Site-specific integration offers a rational strategy for appending new traits to existing loci.
  • Reduces the number of segregating transgenic loci by enabling simultaneous trait introduction.

Conclusions:

  • Site-specific recombinases provide an efficient approach for gene stacking in crop improvement.
  • This method bypasses the need for extensive introgression and separate regulatory approvals for each event.