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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 1, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Multiplexed microsatellite recovery using massively parallel sequencing.

T N Jennings1, B J Knaus, T D Mullins

  • 1Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, OR 97331, USA.

Molecular Ecology Resources
|June 17, 2011
PubMed
Summary
This summary is machine-generated.

Massively parallel sequencing (MPS) offers an inexpensive and accurate genotyping method for natural populations. This approach significantly reduces costs for developing microsatellite markers, aiding conservation efforts for endangered species.

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

  • Genomics
  • Conservation Biology
  • Molecular Ecology

Background:

  • Accurate and affordable genotyping is crucial for managing natural populations.
  • Traditional microsatellite (simple sequence repeat - SSR) analysis is a widely used, cost-effective genotyping method.
  • Advancements in sequencing technology enable new approaches for marker discovery.

Purpose of the Study:

  • To evaluate the efficacy of using multiplexed massively parallel sequencing (MPS) for identifying microsatellite markers.
  • To assess the cost-effectiveness and conversion rates of MPS for microsatellite genotyping.
  • To determine the feasibility of applying this method for large-scale conservation initiatives.

Main Methods:

  • Preparation of seven multiplexed, barcoded, microsatellite-enriched genomic libraries from diverse taxa (conifers and birds).
  • Sequencing of libraries using Illumina Genome Analyzer with paired-end 80-bp reads.
  • Analysis of millions of sequences to identify microsatellite-containing reads and assess marker conversion rates.

Main Results:

  • Screened 6.1 million sequences, identifying 356,958 unique microsatellite-containing microreads.
  • Achieved favorable conversion rates from raw sequences to polymorphic markers compared to existing methods (Sanger, 454).
  • Reduced per-species preparation and sequencing costs to under $400 USD through multiplexed MPS.

Conclusions:

  • Multiplexed MPS provides an accurate, inexpensive, and high-throughput method for microsatellite marker discovery.
  • This approach significantly lowers genotyping costs, making it viable for conserving numerous threatened and endangered species.
  • The method holds substantial promise for advancing population genetics and conservation management.