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Protein kinase C subtypes in tissues derived from neural crest
Y Koda1, H Kobayashi, T Mizuki
1Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Brain Research
|June 4, 1990
Summary
Protein kinase C (PKC) type III is prevalent in neural crest tissues, including adrenal medulla and ganglia. This suggests PKC type III plays a key role in regulating catecholamine synthesis and release.
Area of Science:
- Neuroscience
- Biochemistry
Background:
- Protein kinase C (PKC) encompasses various subtypes with distinct cellular functions.
- Neural crest cells differentiate into diverse cell types, including those in the adrenal medulla and sympathetic ganglia.
- Understanding PKC subtype distribution is crucial for elucidating catecholamine regulation.
Purpose of the Study:
- To determine the subtypes of protein kinase C (PKC) present in neural crest-derived tissues.
- To investigate the role of specific PKC subtypes in the regulation of catecholamine synthesis and release.
Main Methods:
- Analysis of PKC subtypes in bovine adrenal medulla and rat superior cervical ganglia.
- Examination of PKC subtypes in PC12 cells, with and without nerve growth factor treatment.
Main Results:
- Type III PKC was identified as the major subtype in both bovine adrenal medulla and rat superior cervical ganglia.
- A small amount of type II PKC was also detected in these tissues.
- In PC12 cells, type III was predominant, with an additional distinct minor peak observed.
- These findings indicate the prevalence of type III PKC across different neural crest differentiation stages.
Conclusions:
- Type III protein kinase C (PKC) is a major subtype in neural crest-derived tissues.
- PKC type III is likely the key enzyme regulating catecholamine synthesis and release in these cells.