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Related Experiment Video

Updated: May 31, 2026

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
11:41

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Published on: March 10, 2015

Prion seeded conversion and amplification assays.

Christina D Orrú1, Byron Caughey

  • 1Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.

Topics in Current Chemistry
|June 17, 2011
PubMed
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Researchers have advanced cell-free prion conversion reactions, crucial for understanding prion diseases. These developments offer new diagnostic tools for detecting prions and diagnosing transmissible spongiform encephalopathies.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Transmissible spongiform encephalopathies (TSEs) are linked to the misfolding of normal prion protein (PrP(C)) into infectious prions.
  • Prion propagation involves the conversion of PrP(C) into its abnormal isoform.

Purpose of the Study:

  • To provide an overview of cell-free prion conversion and amplification reactions.
  • To discuss the practical and fundamental consequences of these reaction developments.

Main Methods:

  • Development of cell-free prion conversion reactions.
  • Establishment of continuous prion-induced conversion and amplification assays.

Main Results:

  • Significant progress in developing efficient prion conversion and amplification reactions.

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Related Experiment Videos

Last Updated: May 31, 2026

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
11:41

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Published on: March 10, 2015

Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
09:50

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

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  • Gained insights into the molecular mechanisms of prion propagation.
  • Enabled development of ultra-sensitive prion detection and disease diagnostic tests.

    • Conclusions:

    • Cell-free prion conversion reactions are pivotal for understanding prion diseases.
    • These reactions have led to advancements in prion diagnostics and fundamental prion biology research.