Related Experiment Video

Updated: May 31, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

Identification of RUNX3 as a component of the MST/Hpo signaling pathway

Boram Min1, Min-Kyu Kim, Joo-Won Zhang

  • 1Department of Biochemistry, School of Medicine, Chungbuk National University, Cheongju, South Korea.

Journal of Cellular Physiology
|June 17, 2011
PubMed

Insights

The Hippo-MST pathway regulates cell death and proliferation. This study reveals RUNX3 as a key tumor suppressor in this pathway, crucial for its cancer-fighting function.

Area of Science:

  • Molecular Biology
  • Cancer Genetics
  • Cell Biology

Background:

  • The Hippo (Hpo) pathway regulates cell proliferation and death in flies.
  • Its human homolog, the MST pathway, is linked to cancer development.
  • The connection between the MST pathway and tumor suppressor networks is not well understood.

Purpose of the Study:

  • To investigate the role of RUNX3 in the MST pathway.
  • To elucidate the molecular mechanisms linking RUNX3 to MST-mediated tumor suppression.

Main Methods:

  • Genetic screens in fly mutants
  • Molecular analysis
  • siRNA-mediated knockdown

Main Results:

  • RUNX3 is identified as a conserved component of the MST pathway.
  • SAV1/WW45 mediates the interaction between MST2 and RUNX3.
  • RUNX3 knockdown inhibits MST/Hpo-induced cell death.
  • RUNX3 cooperates with MST and SAV1 to induce cell death.

Conclusions:

  • RUNX3 is an endpoint effector of the MST pathway.
  • A novel, evolutionarily conserved regulatory loop involving RUNX3, MST, and SAV1 contributes to tumor suppression.

More Related Videos

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish05:41

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Published on: January 28, 2020

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Pleiotropy01:33

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...