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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

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Related Experiment Video

Updated: May 31, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Proteomics moves from expression to turnover: update and future perspective.

Mary K Doherty1, Phillip D Whitfield

  • 1Department of Diabetes and Cardiovascular Science, University of the Highlands and Islands, Centre for Health Science, Old Perth Road, Inverness, IV2 3JH, UK. mary.doherty@uhi.ac.uk

Expert Review of Proteomics
|June 18, 2011
PubMed
Summary

This study reviews advanced methods for measuring protein synthesis and degradation across the entire proteome. It covers mass spectrometry and other techniques, addressing challenges and bioinformatics needs for analyzing complex proteomic data.

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Area of Science:

  • Proteomics and systems biology.

Background:

  • Understanding protein dynamics is crucial for a holistic view of biological systems.
  • Proteomics aims to elucidate the roles of proteins in biological contexts.

Purpose of the Study:

  • To outline recent advances in experimental strategies for measuring protein synthesis and degradation.
  • To discuss mass spectrometry and non-mass spectrometric approaches in proteomic analysis.

Main Methods:

  • Review of experimental strategies for proteome-wide protein synthesis and degradation measurement.
  • Discussion of mass spectrometry-based and non-mass spectrometry-based approaches.
  • Exploration of bioinformatic resource development for complex proteomic datasets.

Main Results:

  • Recent advances in experimental strategies for measuring protein synthesis and degradation have been outlined.
  • The application of mass spectrometry and non-mass spectrometric approaches is discussed.
  • Challenges in proteomic analyses and the need for bioinformatic resources are explored.

Conclusions:

  • Accurate measurement of protein synthesis and degradation is key to advancing proteomic research.
  • Overcoming analytical challenges requires robust bioinformatic tools for interpreting complex proteomic data.