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Updated: May 31, 2026

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells

Published on: March 5, 2019

Adipose stem cells originate from perivascular cells.

Xiaoxiao Cai¹, Yunfeng Lin, Peter V Hauschka

¹Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.

Biology of the Cell

|June 18, 2011

Summary

Adipose stem cells originate from perivascular cells, identified by α-SMA expression. These cells, found around blood vessels, possess multilineage differentiation and vascularization capabilities, unlike their negative counterparts.

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Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue

Published on: March 2, 2020

Area of Science:

Cell Biology
Stem Cell Research
Tissue Engineering

Background:

Adipose tissues are rich in mesenchymal stem cells (MSCs), but their origin and location are unclear.
Understanding adipose stem cell (ASC) origins is crucial for their purification and study.
Pericytes, marked by α-smooth muscle actin (α-SMA), are potential sources of stem cells.

Purpose of the Study:

To investigate the origin and characteristics of adipose stem cells.
To determine if α-SMA-positive pericytes are the source of ASCs.
To evaluate the differentiation and vascularization potential of identified ASCs.

Main Methods:

Harvesting ASCs from α-SMA-green fluorescent protein (GFP) transgenic mice.
Sorting cells into GFP-positive and GFP-negative populations using fluorescence-activated cell sorting (FACS).
Assessing multilineage differentiation potential, immunofluorescent staining for α-SMA and PDGF-Rβ, and in vitro/in vivo vascularization assays.

Main Results:

α-SMA-GFP-positive cells were located around blood vessels in adipose tissue.
Only α-SMA-GFP-positive cells exhibited multilineage differentiation; negative cells were adipogenic.
α-SMA-GFP-positive cells promoted endothelial cell vascularization in vitro and in vivo.

Conclusions:

Adipose stem cells originate from perivascular cells (α-SMA-positive).
These perivascular cells are located around blood vessels in adipose tissue.
ASCs possess multilineage differentiation and vascularization potential, originating from pericytes.