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Related Experiment Videos

Human papillomavirus type 16 DNA expresses a replication modulation factor in cos-1 cells.

E M Hecht1, P M Chesters, D J McCance

  • 1Department of Microbiology, U.M.D.S. Guy's Campus, London Bridge, U.K.

The Journal of General Virology
|August 1, 1990
PubMed
Summary

Simian virus 40 (SV40) plasmids replicate uncontrollably in cells. Linking these plasmids with human papillomavirus type 16 (HPV-16) DNA prevents this runaway replication, demonstrating HPV-16

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Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • Simian virus 40 (SV40) origin of replication initiates uncontrolled plasmid replication in cos-1 cells.
  • SV40 large T antigen production drives this runaway replication process.

Purpose of the Study:

  • To investigate methods for controlling SV40 plasmid replication.
  • To determine if human papillomavirus type 16 (HPV-16) DNA can regulate SV40 replication.

Main Methods:

  • Covalent linkage of plasmid DNA containing the SV40 origin of replication with HPV-16 DNA.
  • Replication assays in cos-1 cells to monitor plasmid behavior.
  • Analysis of HPV-16 DNA sequences involved in replication control.

Main Results:

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  • Covalent linkage of SV40 plasmids with HPV-16 DNA successfully prevented runaway replication.
  • Replication control was dependent on specific HPV-16 DNA sequences, including the E1 open reading frame and adjacent non-coding regions.

Conclusions:

  • HPV-16 DNA sequences can exert regulatory control over SV40 plasmid replication.
  • The E1 open reading frame and associated non-coding regions of HPV-16 are critical for this regulatory function.