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Human papillomavirus type 16 DNA expresses a replication modulation factor in cos-1 cells
E M Hecht1, P M Chesters, D J McCance
1Department of Microbiology, U.M.D.S. Guy's Campus, London Bridge, U.K.
The Journal of General Virology
|August 1, 1990
Summary
Simian virus 40 (SV40) plasmids replicate uncontrollably in cells. Linking these plasmids with human papillomavirus type 16 (HPV-16) DNA prevents this runaway replication, demonstrating HPV-16
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Simian virus 40 (SV40) origin of replication initiates uncontrolled plasmid replication in cos-1 cells.
- SV40 large T antigen production drives this runaway replication process.
Purpose of the Study:
- To investigate methods for controlling SV40 plasmid replication.
- To determine if human papillomavirus type 16 (HPV-16) DNA can regulate SV40 replication.
Main Methods:
- Covalent linkage of plasmid DNA containing the SV40 origin of replication with HPV-16 DNA.
- Replication assays in cos-1 cells to monitor plasmid behavior.
- Analysis of HPV-16 DNA sequences involved in replication control.
Main Results:
- Covalent linkage of SV40 plasmids with HPV-16 DNA successfully prevented runaway replication.
- Replication control was dependent on specific HPV-16 DNA sequences, including the E1 open reading frame and adjacent non-coding regions.
Conclusions:
- HPV-16 DNA sequences can exert regulatory control over SV40 plasmid replication.
- The E1 open reading frame and associated non-coding regions of HPV-16 are critical for this regulatory function.