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Increased expression of a disintegrin and metalloprotease 8 in allergic rhinitis
Il-Ho Park1, Seong Woong Choi, Hyuk Choi
1Department of Otorhinolaryngology-Head and Neck Surgery, Guro Hospital, Korea University College of Medicine, Seoul, South Korea.
Background:
A disintegrin and metalloproteases (ADAMs) constitute a family of cell surface proteins containing disintegrin and metalloprotease domain, which associate features of adhesion molecules and proteases. ADAM8 is a member of the ADAM family and has recently been implicated to have a role in allergic lung inflammation. Therefore, our aims were to determine the expression of ADAM8 mRNA and protein and to localize ADAM8 protein in the nasal mucosa of patients with allergic rhinitis and control subjects.
Methods:
Inferior turbinate mucosa samples were obtained from 20 patients with allergic rhinitis and 20 matched healthy normal controls. ADAM8 mRNA was extracted from the inferior turbinate mucosa, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in the level of ADAM8 protein expression between patients with allergic rhinitis and normal controls, and the ADAM8 protein was localized with immunohistochemical staining.
Results:
The level of expression of ADAM8 mRNA and protein in the nasal mucosa was significantly increased in patients with allergic rhinitis compared with normal controls. ADAM8 protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands.
Conclusion:
ADAM8 is expressed in human nasal mucosa and is increased in patients with allergic rhinitis. These results suggest a possible contribution for ADAM8 in chronic inflammation of the nasal mucosa in patients with allergic rhinitis.
Insights
Elevated ADAM8 (a disintegrin and metalloprotease domain) protein and mRNA were found in the nasal lining of allergic rhinitis patients. This suggests ADAM8 may play a role in chronic nasal inflammation.
Area of Science:
- Immunology
- Molecular Biology
- Otorhinolaryngology
Background:
- A disintegrin and metalloproteases (ADAMs) are cell surface proteins with adhesion and protease functions.
- ADAM8, a member of the ADAM family, has been linked to allergic lung inflammation.
- The role of ADAM8 in allergic rhinitis has not been fully elucidated.
Purpose of the Study:
- To investigate the expression and localization of ADAM8 mRNA and protein in the nasal mucosa of patients with allergic rhinitis.
- To compare ADAM8 expression levels between allergic rhinitis patients and healthy controls.
Main Methods:
- Inferior turbinate mucosa samples were collected from 20 allergic rhinitis patients and 20 controls.
- ADAM8 mRNA expression was analyzed using reverse transcription-polymerase chain reaction.
- ADAM8 protein levels were assessed by Western blot and immunohistochemical staining.
Main Results:
- ADAM8 mRNA and protein expression were significantly higher in allergic rhinitis patients compared to controls.
- ADAM8 protein was detected in the nasal epithelium, inflammatory cells, and submucosal glands.
- These findings indicate increased ADAM8 in allergic nasal inflammation.
Conclusions:
- ADAM8 is present in human nasal mucosa.
- ADAM8 expression is upregulated in patients suffering from allergic rhinitis.
- ADAM8 may contribute to the chronic inflammation characteristic of allergic rhinitis.
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