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Updated: May 31, 2026

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
06:41

Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

Molecular epidemiology of mumps virus strains circulating in south-west China from 2007 to 2009

Haijing Shi1, Longding Liu1, Shaohui Ma1

  • 1Institute of Medical Biology, Chinese Academy of Medicine Science, Kunming 650118, PR China.

Journal of Medical Microbiology
|June 18, 2011
PubMed

Insights

Genetic analysis of mumps virus (MuV) in China revealed genotype F strains with minor variations. While most genes showed high similarity, the fusion protein displayed geographical differences, aiding future MuV surveillance.

Area of Science:

  • Virology
  • Molecular Biology
  • Epidemiology

Background:

  • Mumps virus (MuV) causes sporadic outbreaks globally.
  • Understanding MuV genetic diversity is crucial for effective control strategies.
  • Previous molecular surveillance data from southwest China was limited.

Purpose of the Study:

  • To investigate the genetic characteristics of mumps virus strains in southwest China.
  • To determine the genotype and genetic variability of isolated MuV strains.
  • To provide data for molecular surveillance and monitoring of MuV circulation.

Main Methods:

  • Nested RT-PCR was used for MuV detection in 117 suspected cases.
  • Sequencing and analysis of the small hydrophobic (SH) gene from 33 isolates.
  • Comparative analysis of haemagglutinin-neuraminidase (HN) and nucleoprotein (N) genes.

Main Results:

  • All 33 investigated MuV isolates belonged to genotype F.
  • High nucleotide and amino acid similarity (>99%) was observed in HN and N genes.
  • The fusion (F) protein showed some geographical variations among the isolates.

Conclusions:

  • Mumps virus strains in southwest China predominantly belong to genotype F.
  • Genetic stability in HN and N genes contrasts with geographical variations in the F protein.
  • This molecular surveillance data is vital for tracking MuV transmission dynamics in China.

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