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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

Seasonal biodiversity of pathogenic fungi in farming air area. Case study

Kinga Plewa1, Elzbieta Lone

  • 1Department of Microbial Ecology and Environmental Protection, Institute of Genetics and Microbiology, University of Wrocław, 63/77 Przybyszewskiego Street, 51-148 Wrocław, Poland. kinga.plewa@microb.uni.wroc.pl

Wiadomosci Parazytologiczne
|June 21, 2011
PubMed

Insights

Poultry farms release airborne fungi, posing health risks like allergies and infections. Fungal abundance varied seasonally, peaking in warmer months, with many species identified as potential respiratory allergens.

Area of Science:

  • Environmental microbiology
  • Occupational health
  • Mycology

Background:

  • Poultry production is a major source of bioaerosols.
  • High concentrations of airborne microorganisms can lead to respiratory irritations, infections, allergies, and toxic effects.

Purpose of the Study:

  • To investigate the seasonal biodiversity of airborne fungi in poultry houses and surrounding areas.
  • To assess the potential health risks associated with airborne fungal exposure in poultry environments.

Main Methods:

  • Seasonal air sampling conducted in spring, summer, autumn, and winter 2010.
  • Air samples collected using Merck MAS-100, cultured on Sabouraud agar, and incubated at 26°C for 5 days.
  • Colony-forming units (CFU) determined, and fungal species identified using standard procedures.

Main Results:

  • Fungal abundance was higher in surrounding areas during summer and autumn compared to spring and winter.
  • A total of 26 fungal species were identified across 12 genera (e.g., Aspergillus, Penicillium, Fusarium).
  • Ten species were found in the poultry house, and 17 in the surrounding areas.

Conclusions:

  • Seasonal variations significantly influence airborne fungal biodiversity in poultry environments.
  • Most identified fungal genera, including Aspergillus and Penicillium, are potential respiratory allergens.
  • Findings highlight the need for risk assessment and management strategies for airborne fungi in poultry production.

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