Toxicologic screening of some surfactants using modern in vivo bioassays

This paper aims to evaluate the degree of skin irritation using specific in vivo tests. The completion of the study is to develop models with wide applicability in toxicological area. HET-CAM or chorioallantoic membrane assay is a new method accepted as an INVITTOX protocol that is a substitute of Draize test. The methods applied in present study were CAM assay on embryonated egg and CD1 Nu/Nu experimental model. The evaluation of erythema that is an important toxic effect of surfactants was done using a Mexameter MX18 (Courage Khazaka research line). The main observations were that sodium lauryl sulphate is the most toxic compound on our series but the non-ionic surfactants are not completely non-noxious. Non-invasive methods can be associated with other test such as CAM assay to evaluate irritant compounds.