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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Sesquiterpene glycosides from Cosmospora joca
Tzong-Huei Lee1, Chung-Kuang Lu, Guei-Jane Wang
1Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan 110, Republic of China. thlee@tmu.edu.tw
Journal of Natural Products
|June 21, 2011
Summary
Five new compounds, cosmosporasides A-E, were isolated from Cosmospora joca. These compounds, except for one, showed potential in inhibiting nitric oxide production in immune cells.
Area of Science:
- Natural Products Chemistry
- Pharmacology
Background:
- Cosmospora joca is a fungus known for producing bioactive secondary metabolites.
- Sesquiterpene glycosides represent a class of natural products with diverse biological activities.
Purpose of the Study:
- To isolate and characterize novel sesquiterpene glycosides from Cosmospora joca.
- To evaluate the anti-inflammatory potential of the isolated compounds by assessing their inhibitory effects on nitric oxide production.
Main Methods:
- Isolation of compounds using ethyl acetate extraction of fermented broth.
- Structure elucidation through comprehensive spectroscopic analyses (NMR, MS) and chemical methods.
- Assessment of nitric oxide inhibition in lipopolysaccharide-activated RAW264.7 cells.
Main Results:
- Five novel sesquiterpene glycosides, cosmosporasides A-E (1-5), were successfully isolated and structurally characterized.
- Compounds 1-5, with the exception of compound 3, demonstrated significant inhibition of nitric oxide production at 100 μM.
- Cosmosporaside 4 exhibited the most potent inhibitory effect, with a median inhibitory concentration (IC50) of 65.98 ± 0.53 μM.
Conclusions:
- The study successfully identified new sesquiterpene glycosides from Cosmospora joca.
- Cosmosporasides A, B, D, and E possess anti-inflammatory properties by inhibiting nitric oxide production, suggesting their potential as therapeutic agents.
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