Parinaric acid methyl ester polymer films with hill-structured features: fabrication and different sensitivities to...

Parinaric acid methyl ester (PnA-Me) polymer films with hill-structured features were fabricated by a solvent volatilization in situ cross-linking method. Moreover, nuclear magnetic resonance, Fourier transform infrared, and oxidation kinetic analyses were successfully applied to monitor the formation process of PnA-Me polymer films. The role of PnA-Me monomer concentrations for growth control of the hill structures on a glass matrix had also been investigated. Also, the results demonstrated that size control of the resulting hill structure ranging from 0.56 ± 0.18 to 19.6 ± 3.5 μm could be realized by varying the concentration of the PnA-Me monomer from 0.0117 to 1.5 mg/mL. Additionally, the effects of polymer films with different surface topographical structures on the behaviors of rat mesenchymal stem cells and human pheochromocytoma cells were measured by morphological and metabolic methods. The results revealed that the cell activity of PnA-Me films was topographical structure- and cell-type-dependent. Furthermore, the selective sensitivity of the PnA-Me films to normal and tumor cells supported the potential value as the coatings for the tissue engineering substitutes.