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Effect of medium/ω-6 long chain triglyceride-based emulsion on leucocyte death and inflammatory gene expression
M F Cury-Boaventura1, R Gorjão, T Martins de Lima
1Institute of Physical Activity and Sports Sciences, Cruzeiro do Sul University, São Paulo, SP, Brazil. maria.boaventura@cruzeirodosul.edu.br
Abstract:
Lipid emulsion (LE) containing medium/ω-6 long chain triglyceride-based emulsion (MCT/ω-6 LCT LE) has been recommended in the place of ω-6 LCT-based emulsion to prevent impairment of immune function. The impact of MCT/ω-6 LCT LE on lymphocyte and neutrophil death and expression of genes related to inflammation was investigated. Seven volunteers were recruited and infusion of MCT/ω-6 LCT LE was performed for 6 h. Four volunteers received saline and no change was found. Blood samples were collected before, immediately afterwards and 18 h after LE infusion. Lymphocytes and neutrophils were studied immediately after isolation and after 24 and 48 h in culture. The following determinations were carried out: plasma-free fatty acids, triacylglycerol and cholesterol concentrations, plasma fatty acid composition, neutral lipid accumulation in lymphocytes and neutrophils, signs of lymphocyte and neutrophil death and lymphocyte expression of genes related to inflammation. MCT/ω-6 LCT LE induced lymphocyte and neutrophil death. The mechanism for MCT/ω-6 LCT LE-dependent induction of leucocyte death may involve changes in neutral lipid content and modulation of expression of genes related to cell death, proteolysis, cell signalling, inflammatory response, oxidative stress and transcription.
Insights
Medium/ω-6 long chain triglyceride-based emulsion (MCT/ω-6 LCT LE) was investigated for its impact on immune cells. This lipid emulsion induced lymphocyte and neutrophil death, potentially through altered gene expression and lipid content.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Medium/ω-6 long chain triglyceride-based emulsion (MCT/ω-6 LCT LE) is recommended to prevent immune function impairment.
- The effects of MCT/ω-6 LCT LE on immune cell viability and inflammatory gene expression require investigation.
Purpose of the Study:
- To investigate the impact of MCT/ω-6 LCT LE on lymphocyte and neutrophil death.
- To examine the effect of MCT/ω-6 LCT LE on the expression of inflammation-related genes in lymphocytes.
Main Methods:
- Seven volunteers received MCT/ω-6 LCT LE infusion; four received saline as a control.
- Blood samples were collected pre-infusion, immediately post-infusion, and 18 hours after.
- Lymphocyte and neutrophil death, neutral lipid accumulation, and gene expression were analyzed post-infusion and after 24/48 hours in culture.
Main Results:
- MCT/ω-6 LCT LE infusion led to increased lymphocyte and neutrophil death compared to saline.
- Changes in plasma fatty acids, triacylglycerol, and cholesterol concentrations were observed.
- Modulation of gene expression related to cell death, inflammation, and transcription was detected in lymphocytes.
Conclusions:
- MCT/ω-6 LCT LE induces lymphocyte and neutrophil death.
- Mechanisms may involve altered neutral lipid content and gene expression modulation.
- Further research is needed to understand the full implications for immune function.