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Updated: May 31, 2026

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
Published on: October 21, 2016
Cationic polyacrylamides promote binding of cellulase and amylase
John T Reye1, Kendra E Maxwell, Sujit Banerjee
1Institute of Paper Science and Technology, School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 500 10th Street, NW, Atlanta, GA 30332-0620, United States.
Abstract:
Cationic polyacrylamides (c-PAMs) increase the binding of cellulase to cellulose and of α-amylase to cornstarch. The c-PAMs increase the rate of hydrolysis of both substrates by about the same amount, which suggests that the increased binding is responsible for the enhanced rates. These results support our proposed mechanism where the c-PAM neutralizes the charge of the substrate through a "patching" mechanism, which reduces the charge repulsion between fiber and enzyme.
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