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Updated: May 31, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Assessment of a bioaugmentation strategy with polyphosphate accumulating organisms in a nitrification/denitrification
Carlota Tayà1, Albert Guisasola, Juan A Baeza
1Departament d'Enginyeria Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. Carlota.Taya@uab.cat
Abstract:
Different alternative configurations and strategies for the simultaneous biological removal of organic matter and nutrients (N and P) in wastewater have been proposed in the literature. This work demonstrates a new successful strategy to bring in enhanced biological phosphorus removal (EBPR) to a conventional nitrification/denitrification system by means of bioaugmentation with an enriched culture of phosphorus accumulating organisms (PAO). This strategy was tested in a sequencing batch reactor (SBR), where an 8h configuration with 3h anoxic, 4.5h aerobic and 25 min of settling confirmed that nitrification, denitrification and PAO activity could be maintained for a minimum of 60 days of operation after the bioaugmentation step. The successful bioaugmentation strategy opens new possibilities for retrofitting full-scale WWTP originally designed for only nitrification/denitrification. These systems could remove P simultaneously to COD and N if they were bioaugmented with waste purge of an anaerobic/aerobic SBR operated in parallel treating part of the influent wastewater.
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