Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modified graphene oxide nanoplates reinforced 3D printed multifunctional scaffold for bone tissue engineering.

Biomaterials advances·2022
Same author

5FU encapsulated polyglycerol sebacate nanoparticles as anti-cancer drug carriers.

RSC advances·2022
Same author

Detection of 16S rRNA gene for rapid identification of bacterial pathogens causing peritonitis in patients on continuous ambulatory peritoneal dialysis.

Indian journal of medical microbiology·2022
Same author

Correction to: Cardiac Differentiation of Mesenchymal Stem Cells: Impact of Biological and Chemical Inducers.

Stem cell reviews and reports·2021
Same author

Cardiac Differentiation of Mesenchymal Stem Cells: Impact of Biological and Chemical Inducers.

Stem cell reviews and reports·2021
Same author

Bacterial diversity and functional analysis of severe early childhood caries and recurrence in India.

Scientific reports·2020
Same journal

Lysozyme assay using a rationally designed GN4G2 substrate with coupled β-glucosidase reaction.

Analytical biochemistry·2026
Same journal

The long run: A tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
08:07

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Scanning electron microscopy preparation protocol for differentiated stem cells.

Sreejit Parameswaran1, Rama S Verma

  • 1Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, TN, India.

Analytical Biochemistry
|June 21, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a new scanning electron microscopy (SEM) protocol for preparing adult bone marrow-derived mesenchymal stem cells (BMSC) undergoing adipogenesis. The method preserves cellular structures for accurate morphological analysis.

More Related Videos

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
09:15

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Published on: August 16, 2019

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
09:03

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

Related Experiment Videos

Last Updated: May 31, 2026

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
08:07

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
09:15

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy

Published on: August 16, 2019

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
09:03

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Published on: May 19, 2023

Area of Science:

  • Cell Biology
  • Biotechnology
  • Microscopy

Background:

  • Scanning electron microscopy (SEM) is crucial for visualizing cellular morphology.
  • Established SEM protocols for adipogenic differentiation of stem cells are lacking.
  • Preserving lipid deposits during sample preparation is challenging.

Purpose of the Study:

  • To develop a novel SEM protocol for differentiated adult bone marrow-derived mesenchymal stem cells (BMSC).
  • To maintain the structural integrity of cellular components, especially lipid deposits, during SEM sample preparation.
  • To enable accurate morphological and physical characterization of stem cell differentiation.

Main Methods:

  • Developed a protocol involving cell fixation, dehydration with methanol, and vacuum desiccation.
  • Avoided long-chain alcohols to prevent lipid dissolution.
  • Omitted osmium tetroxide postfixation and critical point drying to reduce processing time.

Main Results:

  • The protocol successfully preserved the structural organization of differentiated BMSC.
  • Lipid deposits within the cells remained intact, allowing for detailed morphological analysis.
  • Sample processing time was significantly reduced compared to traditional methods.

Conclusions:

  • The developed SEM protocol is effective for characterizing adipogenic differentiation in stem cells.
  • This method facilitates the assessment of stem cell potential, fate, and differentiation degree.
  • The protocol is particularly useful for analyzing stem cells cultured on various scaffolds.