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Updated: May 31, 2026

Preparation of Meiotic Chromosome Spreads from Mouse Oocytes for Assessment of Synapsis and Recombination
Published on: July 18, 2025
[Chromosome recombination in rice meiosis]
Jun Chen1, Wei-Xiong Luo, Ming Li
1Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management/ Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China. haminychen@163.com
Abstract:
Meiosis is a highly conservative process, which plays important role in the life cycles of all sexually reproductive organisms, while the pairing, synapsis, and recombination are the key events in this process and have become the hotspots in meiosis studies. At present, we cannot observe the process of cross and recombination of chromosomes directly in plant meiosis, and generally conclude the process by analysis the genetic population. In the present study, we analyzed 32 DH lines using graphical genotypes, and found 4 chromosomes out of 32 DH lines had regional heterozygosis, which was further confirmed using STS markers. We suggested that it may cause by repair incompletion or mis-repair of chromsome. These results provid some directly evidence for explaining the mechanism of plant meiosis.
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