Related Experiment Video
Updated: May 31, 2026
Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
Published on: October 4, 2024
Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori)
Sai Zhang1, Yunmin Xu, Qiang Fu
1The Key Sericultural Laboratory of Agricultural Ministry, College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
Abstract:
The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were identified in this study; and among them, 69 proteins were observed in midgut for the first time. We also found that the silkworm larval midgut responded to starvation by producing a 10 kDa heat shock protein and a diapause hormone precursor.
