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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
Do infections induce monoclonal immunoglobulin components?
H Haas1, S Anders, G W Bornkamm
1Research Institute, Borstel, FRG.
Clinical and Experimental Immunology
|September 1, 1990
Summary
Monoclonal immunoglobulins (MC) were incidentally found in infections like visceral leishmaniasis and CMV. Further research is needed to understand the high incidence of MC in these specific infections.
Area of Science:
- Infectious Diseases
- Immunology
- Clinical Chemistry
Background:
- Monoclonal immunoglobulins (MC) are sometimes incidentally found in patients with infections.
- The prevalence and significance of MC in various infectious diseases require systematic investigation.
Purpose of the Study:
- To systematically investigate the presence of monoclonal immunoglobulin components (MC) in patients with specific infections.
- To determine the incidence of MC in visceral leishmaniasis, cytomegalovirus (CMV) infection, echinococcosis, and infectious mononucleosis.
Main Methods:
- Isoelectric focusing with immunoblotting was employed to detect MC.
- The detection limit for MC was established at 0.1 mg/ml.
- Patients with visceral leishmaniasis, CMV, echinococcosis, and infectious mononucleosis were analyzed.
Main Results:
- MC were detected in 16/20 leishmaniasis patients and 8/18 CMV patients.
- Only one echinococcosis patient and no infectious mononucleosis patients showed MC.
- MC were generally transient, and a subset in leishmaniasis patients bound to leishmania antigens.
Conclusions:
- A high incidence of MC was observed in visceral leishmaniasis and CMV infections.
- The specificity of most detected MC remains undetermined.
- Further research is necessary to elucidate the mechanisms behind MC occurrence in CMV and leishmaniasis.
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