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Updated: May 31, 2026

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
Published on: March 28, 2017
Protocol for high-resolution electrophoresis separation of myosin heavy chain isoforms in bovine skeletal muscle
Brigitte Picard1, Christiane Barboiron, David Chadeyron
1INRA, UR1213 Herbivores, Centre de Clermont-Ferrand/Theix, Saint Genès Champanelle, France. brigitte.picard@clermont.inra.fr
Abstract:
In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.
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