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Published on: December 28, 2013
Quantitative tissue oxygen measurement in multiple organs using 19F MRI in a rat model
Siyuan Liu1, Sameer J Shah, Lisa J Wilmes
1Department of Radiology and Biomedical Imaging, University of California, San Francisco, California 94143-0464, USA.
Magnetic Resonance in Medicine
|June 21, 2011
Summary
This study shows that 19F MRI using hexafluorobenzene can measure oxygen levels in various rat organs. Hyperoxia significantly increased tissue oxygen partial pressure (ptO2) across multiple organs.
Area of Science:
- Biomedical Imaging
- Physiology
- Magnetic Resonance Imaging
Background:
- Measuring organ tissue oxygen levels is crucial for evaluating medical interventions like wound healing and cancer therapeutics.
- Previous 19F MRI applications focused on low tumor oxygen levels (pO2 < 30 mmHg).
- The technique relies on the linear relationship between hexafluorobenzene (HFB) relaxation rate (R1) and pO2.
Purpose of the Study:
- To investigate the feasibility of 19F MRI for measuring a wider range of pO2 values.
- To assess the measurement of individual organ tissue pO2 in vivo using a rat model.
- To evaluate the impact of hyperoxia on tissue oxygen levels in multiple organs.
Main Methods:
- In vitro validation of the linear relationship between HFB R1 and pO2 (0-760 mmHg) at various temperatures (25, 37, 41°C) at 7 Tesla.
- In vivo measurement of rat tissue oxygen partial pressure (ptO2) using 19F saturation recovery echo planar imaging.
- Measurement of ptO2 in brain, kidney, liver, gut, muscle, and skin during inhalation of 30% and 100% oxygen.
Main Results:
- A validated linear relationship between HFB R1 and pO2 was confirmed in vitro.
- In vivo experiments demonstrated significant increases in ptO2 across all measured organs under hyperoxic conditions (P < 0.001).
- The technique proved feasible for measuring regional ptO2 in multiple organs.
Conclusions:
- 19F MRI of hexafluorobenzene is a feasible method for in vivo regional ptO2 measurement.
- Hyperoxia significantly elevates ptO2 in various organs in a rat model.
- This technique holds potential for evaluating interventions related to tissue oxygenation.
