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Related Experiment Video

Updated: May 31, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Nonideal mixing in multicomponent lipid/detergent systems.

Alekos Tsamaloukas¹, Halina Szadkowska, Heiko Heerklotz

¹Biozentrum of the University of Basel, Division of Biophysical Chemistry, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.

Journal of Physics. Condensed Matter : an Institute of Physics Journal

|June 22, 2011

Summary

Understanding detergent mixing with lipids is key for protein extraction assays. Triton X-100 addition to lipid mixtures promotes domain separation, influenced by cholesterol interactions, impacting membrane patch composition.

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Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

Published on: September 1, 2023

Area of Science:

Biochemistry
Physical Chemistry
Membrane Biophysics

Background:

Detergent-based assays are crucial for solubilizing and purifying membrane proteins and lipids.
Understanding lipid-detergent interactions is essential for interpreting these assays accurately.
Triton X-100 (TX-100) is a widely used nonionic detergent in membrane biophysics.

Purpose of the Study:

To investigate the mixing properties of binary and ternary lipid mixtures with Triton X-100.
To characterize the partitioning thermodynamics of TX-100 in lipid bilayers.
To analyze the influence of cholesterol on lipid-detergent interactions and domain formation.

Main Methods:

High-sensitivity isothermal titration calorimetry (ITC) was employed.
Thermodynamic modeling was used to analyze composition-dependent partition coefficients.
Binary and ternary lipid mixtures of POPC, SM, and cholesterol were studied.

Main Results:

POPC, fluid SM, and TX-100 exhibit near-ideal mixing.
Favorable SM/cholesterol and unfavorable PC/cholesterol interactions were observed.
TX-100/cholesterol contacts are unfavorable, promoting domain segregation.

Conclusions:

TX-100 addition drives the separation of SM/cholesterol-rich domains from POPC/TX-100-rich domains.
Cholesterol-detergent interactions are critical in determining detergent-resistant membrane patch composition.
This study provides insights into lipid-detergent phase behavior relevant to membrane protein studies.