Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA calorimetric force spectroscopy at single base pair resolution.

Nature communications·2025
Same author

Massively parallel analysis of single-molecule dynamics on next-generation sequencing chips.

Science (New York, N.Y.)·2024
Same author

Variance sum rule for entropy production.

Science (New York, N.Y.)·2024
Same author

Detection of single DNA mismatches by force spectroscopy in short DNA hairpins.

The Journal of chemical physics·2020
Same author

Experimental evidence of symmetry breaking of transition-path times.

Nature communications·2019
Same author

Derivation of the spin-glass order parameter from stochastic thermodynamics.

Physical review. E·2018
Same journal

Interplay of Anisotropy, Dzyaloshinskii Moriya Interaction and Symmetry breaking Fields in a 2D XY Ferromagnet.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Single-molecule electron transport near a charge-trapping orbital-level alignment.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Δ<sub>T</sub>Noise as a Robust Diagnostic for Chiral, Helical and Trivial Edge Modes.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

A Quantum Framework for Negative Magnetoresistance in Multi-Weyl Semimetals.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Magnetic anisotropy and electronic structure in surface-supported single rare-earth atom magnets: a topical review.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same journal

Modeling thermal transport in AlN/GaN superlattices and heterostructures with machine-learned force fields.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
See all related articles

Related Experiment Video

Updated: May 31, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Single-molecule experiments in biological physics: methods and applications.

F Ritort1

  • 1Departament de Física Fonamental, Facultat de Física, Universitat de Barcelona, Diagonal 647, 08028 Barcelona, Spain.

Journal of Physics. Condensed Matter : an Institute of Physics Journal
|June 22, 2011
PubMed
Summary
This summary is machine-generated.

Single-molecule experiments (SMEs) enable precise manipulation and measurement of individual molecules, offering unique insights into biomolecular processes and statistical mechanics. These techniques complement traditional bulk assays by providing detailed thermodynamic and kinetic data.

More Related Videos

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Related Experiment Videos

Last Updated: May 31, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

Area of Science:

  • Biological physics
  • Biophysics
  • Statistical mechanics

Background:

  • Technological advancements enable high-sensitivity instruments for single-molecule manipulation and force measurement.
  • Single-molecule experiments (SMEs) provide insights beyond traditional bulk assays.

Purpose of the Study:

  • To review single-molecule experiments (SMEs) in biological physics.
  • To highlight the importance and interdisciplinary nature of SMEs.
  • To provide an introductory overview for interested scientists.

Main Methods:

  • Atomic-force microscopy (AFM)
  • Laser optical tweezers (LOTs)
  • Magnetic tweezers (MTs)
  • Biomembrane force probes (BFPs)
  • Single-molecule fluorescence (SMF)

Main Results:

  • SMEs allow manipulation of individual molecules, measurement of molecular properties, and characterization of reaction kinetics.
  • These experiments reveal thermodynamics and kinetics of biomolecular processes.
  • SMEs facilitate the study of nonequilibrium thermodynamics and fluctuation theorems.

Conclusions:

  • SMEs offer powerful methods to study biomolecular systems and fundamental physics.
  • The review covers experimental techniques, applications, and future perspectives.
  • SMEs are crucial for understanding molecular behavior and statistical mechanics.