Related Experiment Video
Updated: Jan 31, 2026

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
MicroRNA expression profiling in archival tissue specimens: methods and data processing
Thomas Streichert1, Benjamin Otto, Ulrich Lehmann
1Department of Clinical Chemistry/Central Laboratories, Universitätsklinikum Hamburg-Eppendorf, 20246 Hamburg, Germany.
Abstract:
The discovery that the human genome codes for thousands (if not millions) of previously unrecognized non-protein coding RNAs with regulatory functions has changed our understanding of many physiological and pathological processes. A prominent class of non-coding RNAs with important functions in cancer initiation and progression comprised by very short single-stranded, mRNA translation modulating RNAs, termed microRNAs. The determination of microRNA expression profiles is now widely used in biology and pathology, employing a range of methodologies. A steadily growing number of studies describe the analysis of formalin-fixed paraffin-embedded, so-called "archival" specimens. However, procedures for data processing and calculations are far from standardized and differ considerably between published studies, making comparisons and meta-analyses still quite difficult. In this review, we provide a short overview of profiling methods used for archival samples and describe in detail a modified method for normalization and processing of raw data obtained by fluorescence-labeled bead technology from Luminex.Inc.
Related Concept Videos
Coordination of Gene Expression Processes in Bacteria
Archival Research
MicroRNAs
MicroRNAs
The Scientific Method in Nursing Process
When using research findings to change practice, one must understand the process used to guide a study. The scientific method is a systematic, step-by-step process that supports the data's validity, reliability, and generalizability. As a result, findings can be...
Statistical Methods for Analyzing Epidemiological Data

