Clay occlusion of respirable quartz particles detected by low voltage scanning electron microscopy-X-ray analysis

Respirable-sized dust particles from a clay mine and mill were examined for aluminium and silicon content by scanning electron microscopy-energy dispersive X-ray analysis at incident electron energies of 5-20 keV. Most of the high silica content particles, those which had a silicon to silicon plus aluminium signal fraction, measured at 20 keV, of 0.9 or greater, showed a decrease in the silicon fraction of signal with decreasing incident electron energy, down to values of between 0.6 and 0.8 at 5 keV: this decrease was a function of particle size and composition. Simple models for silicon fraction of signal vs incident electron voltage suggest this behaviour is due to a heterogeneous particle structure in which an aluminosilicate coating occludes the surface of a silica particle core. The coating survives incubation in a major component of pulmonary surfactant.