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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Published on: March 24, 2016
An analytical pipeline for MALDI in-source decay mass spectrometry imaging
Tyler A Zimmerman1, Delphine Debois, Gabriel Mazzucchelli
1Mass Spectrometry Laboratory, GIGA-R Systems Biology and Chemical Biology, University of Liège, Liège (Sart-Tilman), Belgium.
Analytical Chemistry
|June 23, 2011
Summary
Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) mass spectrometry imaging enables direct protein sequencing and localization in tissues. A new analytical pipeline improves efficiency for identifying and mapping proteins within tissue sections.
Area of Science:
- Proteomics
- Mass Spectrometry Imaging
- Biochemistry
Background:
- Matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) enables direct protein sequencing from mass spectra.
- Combining MALDI-ISD with mass spectrometry imaging (MSI) allows simultaneous protein sequencing and spatial localization within tissues.
- Development of novel data analysis tools is crucial for effective MALDI-ISD MSI.
Purpose of the Study:
- To investigate the properties of MALDI-ISD for protein mixtures.
- To develop an analytical pipeline for MALDI-ISD MSI data analysis.
- To demonstrate simultaneous protein identification and localization in tissue sections.
Main Methods:
- Utilized matrix-assisted laser desorption/ionization (MALDI) with 1,5-diaminonaphthalene (DAN) matrix for in-source decay (ISD) mass spectrometry.
- Developed and applied a computational pipeline for analyzing MALDI-ISD mass spectrometry imaging data.
- Analyzed tissue sections from porcine eye lens using the developed pipeline.
Main Results:
- Identified high sensitivity of MALDI-ISD to relative protein ionization efficiency in mixtures.
- Successfully sequenced and localized proteins, including crystallins and cytochrome c, in porcine eye lens tissue sections.
- Demonstrated the pipeline's capability to generate ion images for comparison with tissue morphology.
Conclusions:
- MALDI-ISD MSI, coupled with a robust analytical pipeline, is a powerful tool for simultaneous protein identification and localization in biological tissues.
- The developed pipeline enhances the efficiency of protein discovery and spatial mapping in complex biological samples.
- This approach offers a significant advancement for proteomic analysis directly within tissue contexts.
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