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Updated: May 31, 2026

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
11:22

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Engineering biological-based vascular grafts using a pulsatile bioreactor.

Angela H Huang1, Laura E Niklason

  • 1Department of Biomedical Engineering, Yale University, USA.

Journal of Visualized Experiments : Jove
|June 23, 2011
PubMed
Summary

This study presents a novel bioreactor for regenerating small-diameter arterial bypass grafts. The system mimics physiological conditions, producing functional vascular tissues with mechanical properties suitable for implantation.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Developing functional small-diameter arterial bypass grafts is crucial for cardiovascular therapies.
  • Native arterial vessels require both mechanical and chemical stimulation for proper development.
  • Existing bioreactor systems often lack the ability to fully replicate the native physiological environment.

Purpose of the Study:

  • To present a novel bioreactor system for culturing engineered vascular grafts.
  • To investigate the regeneration of functional small-diameter arterial bypasses under controlled chemo-mechanical conditions.
  • To evaluate the histological and mechanical properties of engineered vessels.

Main Methods:

  • Smooth muscle cells (SMCs) seeded onto polyglycolic acid (PGA) mesh, threaded over silicone tubing.

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Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
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Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

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Last Updated: May 31, 2026

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
11:22

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch
07:51

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

Published on: December 10, 2020

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

  • Culture within a bioreactor with or without pulsatile stimulation for up to 12 weeks.
  • Application of cyclic radial strain to mimic physiological pulsatile flow.

    • Main Results:

    • Engineered vessels exhibit histological resemblance to native blood vessels.
    • Vessel walls express mature smooth muscle myosin heavy chain (SMMHC) and deposit substantial collagen.
    • Pulsatile bioreactor consistently regenerates vessels with mechanical properties suitable for animal implantation.

    Conclusions:

    • The developed bioreactor effectively supports the regeneration of functional small-diameter vascular grafts.
    • The system provides a controlled chemo-mechanical environment, including pulsatile stimulation.
    • This bioreactor serves as an excellent platform for studying vascular regeneration mechanisms.